海森大数据

专利号： US07005279B1

A sugar chain in which N-acetylglucosamine was added to the non-reducing end of the sugar chain (Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc) was synthesized by allowing the FLAG peptide-fused G4 polypeptide produced as a secreted form in Example 10 to react simultaneously with LNnT and β1,4-galactosyltransferase (manufactured by SIGMA) purified from bovine milk. In the same manner, Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc was synthesized using LNT as a substrate. Also, Galβ1-4GlcNAcβ1-3Gal

实验方案

产品
反应物	N-acetylglucosamine Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc ice ice LNnT LNFP-V Galβ1-3GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc
试剂
程序	1、Add A sugar chain in which N-acetylglucosamine was added to the non-reducing end of the sugar chain (Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc) 2、Synthesize was synthesized 3、Yield produced as a secreted form in Example 10 4、Synthesize to react simultaneously with LNnT and β1,4-galactosyltransferase (manufactured by SIGMA) 5、Purify purified from bovine milk 6、Synthesize In the same manner, Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc was synthesized 7、Synthesize Also, Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc was synthesized 8、Synthesize The specific reaction

物质
- Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc
  
  SMILES:O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2[C@H]([C@H](O[C@@H]3[C@H]([C@H](O[C@@H]4[C@H]([C@H](O[C@H]5[C@@H]([C@H](C(O)O[C@@H]5CO)O)O[C@H]5[C@@H](O)[C@H](O)[C@H](O)[C@@H](O5)C)O[C@@H]([C@@H]4O)CO)O)O[C@@H]([C@H]3O)CO)NC(=O)C)O[C@@H]([C@@H]2O)CO)O)O[C@@H]1CO)NC(=O)C)O
  
  inchi:1S/C46H78N2O35/c1-10-21(57)27(63)29(65)43(71-10)83-39-31(67)40(70)72-18(9-54)35(39)79-45-32(68)38(25(61)15(6-51)75-45)82-42-20(48-12(3)56)36(23(59)14(5-50)73-42)80-46-33(69)37(24(60)16(7-52)76-46)81-41-19(47-11(2)55)26(62)34(17(8-53)77-41)78-44-30(66)28(64)22(58)13(4-49)74-44/h10,13-46,49-54,57-70H,4-9H2,1-3H3,(H,47,55)(H,48,56)/t10-,13+,14+,15+,16+,17+,18+,19+,20+,21+,22-,23+,24-,25-,26+,27+,28-,29-,30+,31+,32+,33+,34+,35+,36+,37-,38-,39+,40?,41-,42-,43-,44-,45-,46-/m0/s1
  
  角色：product
  
  分类：产品
  
  实体类型：exact
- N-acetylglucosamine
  
  SMILES:C(C)(=O)N[C@H]1C(O)O[C@@H]([C@H]([C@@H]1O)O)CO
  
  inchi:1S/C8H15NO6/c1-3(11)9-5-7(13)6(12)4(2-10)15-8(5)14/h4-8,10,12-14H,2H2,1H3,(H,9,11)/t4-,5-,6-,7-,8?/m1/s1
  
  角色：reactant
  
  分类：原料
  
  实体类型：exact
- Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc
  
  SMILES:O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2[C@H]([C@H](O[C@H]3[C@@H]([C@H]([C@H](O[C@@H]4[C@H]([C@H](O[C@H]5[C@@H]([C@H](C(O)O[C@@H]5CO)O)O)O[C@@H]([C@@H]4O)CO)O)O[C@@H]3CO)NC(=O)C)O)O[C@@H]([C@@H]2O)CO)O)O[C@@H]1CO)NC(=O)C)O
  
  inchi:1S/C40H68N2O31/c1-9(49)41-17-22(54)30(69-38-27(59)24(56)19(51)11(3-43)64-38)15(7-47)67-36(17)72-33-20(52)12(4-44)65-39(28(33)60)70-31-16(8-48)68-37(18(23(31)55)42-10(2)50)73-34-21(53)13(5-45)66-40(29(34)61)71-32-14(6-46)63-35(62)26(58)25(32)57/h11-40,43-48,51-62H,3-8H2,1-2H3,(H,41,49)(H,42,50)/t11-,12-,13-,14-,15-,16-,17-,18-,19+,20+,21+,22-,23-,24+,25-,26-,27-,28-,29-,30-,31-,32-,33+,34+,35?,36+,37+,38+,39+,40+/m1/s1
  
  角色：reactant
  
  分类：原料
  
  实体类型：exact
- ice
  
  SMILES:O
  
  inchi:1S/H2O/h1H2
  
  角色：reactant
  
  分类：原料
  
  实体类型：chemicalClass
- ice
  
  SMILES:O
  
  inchi:1S/H2O/h1H2
  
  角色：reactant
  
  分类：原料
  
  实体类型：chemicalClass
- LNnT
  
  SMILES:CC(=O)N[C@@H]1[C@H]([C@@H]([C@H](O[C@H]1O[C@H]2[C@H]([C@H](O[C@H]([C@@H]2O)O[C@@H]3[C@H](OC([C@@H]([C@H]3O)O)O)CO)CO)O)CO)O[C@H]4[C@@H]([C@H]([C@H]([C@H](O4)CO)O)O)O)O
  
  inchi:1S/C26H45NO21/c1-6(32)27-11-14(35)20(46-25-18(39)15(36)12(33)7(2-28)43-25)10(5-31)45-24(11)48-22-13(34)8(3-29)44-26(19(22)40)47-21-9(4-30)42-23(41)17(38)16(21)37/h7-26,28-31,33-41H,2-5H2,1H3,(H,27,32)/t7-,8-,9-,10-,11-,12+,13+,14-,15+,16-,17-,18-,19-,20-,21-,22+,23?,24+,25+,26+/m1/s1
  
  角色：reactant
  
  分类：原料
  
  实体类型：exact
- LNFP-V
  
  SMILES:CC1C(C(C(C(O1)OC(C(C=O)O)C(C(CO)O)OC2C(C(C(C(O2)CO)O)OC3C(C(C(C(O3)CO)O)OC4C(C(C(C(O4)CO)O)O)O)NC(=O)C)O)O)O)O
  
  inchi:1S/C32H55NO25/c1-8-16(42)20(46)22(48)30(51-8)55-25(10(40)3-34)26(11(41)4-35)56-32-24(50)28(19(45)14(7-38)54-32)58-29-15(33-9(2)39)27(18(44)13(6-37)52-29)57-31-23(49)21(47)17(43)12(5-36)53-31/h3,8,10-32,35-38,40-50H,4-7H2,1-2H3,(H,33,39)
  
  角色：reactant
  
  分类：原料
  
  实体类型：exact
- Galβ1-3GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc
  
  SMILES:O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)[C@@H]1[C@H]([C@H](O[C@@H]2[C@H]([C@H](O[C@H]3[C@@H]([C@H](C(O)O[C@@H]3CO)O)O[C@H]3[C@@H](O)[C@H](O)[C@H](O)[C@@H](O3)C)O[C@@H]([C@@H]2O)CO)O)O[C@@H]([C@H]1O)CO)NC(=O)C
  
  inchi:1S/C32H55NO25/c1-7-14(39)18(43)20(45)30(50-7)58-27-22(47)28(49)51-12(6-37)24(27)55-32-23(48)26(17(42)11(5-36)54-32)57-29-13(33-8(2)38)25(16(41)10(4-35)52-29)56-31-21(46)19(44)15(40)9(3-34)53-31/h7,9-32,34-37,39-49H,3-6H2,1-2H3,(H,33,38)/t7-,9+,10+,11+,12+,13+,14+,15-,16+,17-,18+,19-,20-,21+,22+,23+,24+,25+,26-,27+,28?,29-,30-,31-,32-/m0/s1
  
  角色：reactant
  
  分类：原料
  
  实体类型：exact

专利号： US07005279B1

实验方案

物质