Dissolve the preservatives in 75 cc of the water which has been heated to boiling. Use 25 cc of this solution while hot to wet the methylcellulose. Cool the remainder of the preservative solution before adding to the wet methylcellulose, shake, and place in a refrigerator until a clear solution of the methylcellulose is obtained. Add the Intracaine hydrochloride which has previously been dissolved in the remaining 25 cc of distilled water and mix thoroughly. Autoclave 15 min at 121'C in 30 cc wide-mouth, tightly capped bottles or in an Erlenmeyer flask covered with glassine paper and gauze. Pour into sterile jars.
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